TABLE 1.

Summary of tests and parameters used in each experiment

Experiment	Test	Tested parameter	Type of arena	Sample size	Variations of FS2
Exp 1	a. Amplitude of the playback	Amplitude of the signal measured as substrate velocity (μm s−1)	Bean plant	42 males	FS2 (0–8000 μm s−1); Control – silence
	b. Continuity of the playback	Continuity of the signal (with or without silent breaks)	Bean plant	20 males	FS2‐continuous – FS2 played in loop with no breaks; FS2‐discontinuous – 11.5 s FS2‐2 playback with breaks of 11 s of silence; Control – silence
	c. Dominance of the harmonics	Dominant frequency (Hz)	Round arena	20 males	FS2‐76 – peak of frequency at the first harmonic (76 Hz); FS2‐even – first and second harmonics equally important; FS2‐152 – peak of frequency at the second harmonic (152 Hz);
	d. Pulse repetition time	PRT (s)	Round arena	50 males	FS2‐fast – PRT at 1.0 s; FS2‐standard – PRT at 1.5 s
Exp 2	a. One‐choice test	Optimal Exp1 features (a, b, c, d)	T arena	59 males	FS2‐Best – FS2 continuous play, 76 Hz and 1 s PRT;
	b. Simple two‐choice test	Dominant frequency (Hz)	T arena	38 males	FS2‐76 – peak of frequency at the first harmonic (76 Hz); FS2‐152 – peak of frequency at the second harmonic (152 Hz);
	c. Complex two‐choice test	Dominant frequency (Hz) and PRT (s)	T arena	80 males	FS2‐Best – FS2 with 100–150 μm s−1, continuous play, 76 Hz and fast PRT; FS2‐Worst – FS2 with 100–150 μm s−1, continuous play, 152 Hz and standard PRT; FS2‐Sub1 – FS2 with 100–150 μm s−1, continuous play, 76 Hz and standard PRT; FS2‐Sub2 – FS2 with 100–150 μm s−1, continuous play, 152 Hz and fast PRT;