TABLE 1.

The effect of platelet stimulation on the levels of PDI enzymes and other selected platelet‐secreted molecules

	Lysate	Releasate
T granules
PDIA1	No change	No change
PDIA3	No change	No change
PDIA4	No change	No change
PDIA5	No change	No change
PDIA6	No change	↓ *
PDIA9	No change	No change
PDIA10	↑ **	No change
PDIA15	No change	No change
α granules
P‐sel	No change	↑ *
PBP	↓ **	No change
PF4	↓ *	↑ **
vWf	↓ ***	No change
TGFβ1	No change	↑ *
FV	↓ **	No change
TSP−1	↓ ***	↑ ***
Plg	‐	↑ ***
PAI−1	‐	↑ *

Lysates and releasates of platelets resting and stimulated with 0.5 U/mL thrombin were analyzed with the LC‐MS/MS method. PDI isoforms and selected platelet‐secreted molecules were semiquantified using emPAI % calculation. ↑ increase; ↓ decrease; *p < .05, **p < .01, ***p < .001; n = 3–6; Student's t test or Mann‐Whitney test. Detailed data are presented in Table S1 in the Supporting information.

Abbreviations: FV, factor V; PAI‐1. plasminogen activator inhibitor 1; P‐sel, P‐selectin; PBP, protein basic protein; PF4, platelet factor 4; Plg, plasminogen; TGFβ1, transforming growth factor β1; TSP‐1, thrombospondin 1; vWf, von Willebrand factor.