FIG. 1.

71D6 binds to MET at high affinity and elicits MET activation and downstream signaling, mimicking HGF function. (A) 71D6 binding to hMET or mMET extracellular domain was analyzed by ELISA (a.u.). (B) MET activation and downstream signaling induced by HGF or 71D6 was studied in human mouse MLP29 liver precursor cells. Cell lysates were analyzed by Western blotting using antibodies specific for the phosphorylated forms of MET, ERK, and AKT (pMET, pERK and pAKT) as well as antibodies against total MET, ERK, and AKT.