FIGURE 2.

(a) Quantitative PCR demonstrating human MBTPS2 expression in stable M19‐CHO cell lines. Mutant MBTPS2 expression levels are normalized to cells expressing wild‐type MBTPS2. GAPDH was used as a normalization control. (b) Immunoblot demonstrating S2P protein expression in stable M19‐CHO cell lines (upper panel) and actin normalization control (lower panel). (c) M19‐CHO cells stably transfected with vector alone (negative control), wild‐type MBTPS2, or mutation‐bearing MBTPS2 (Val256Leu, Arg429His, and Gly500Asp) underwent dual transfection with a luciferase reporter gene driven by a sterol‐responsive element and renilla luciferase driven by a constitutive promoter. Results are pooled from three independent experiments. Error bars represent standard deviation. *p < 0.05; **** p < 0.0001 by two‐way ANOVA with Dunnett's posthoc test