Figure 4.

Chitin‐triggered MAPK activation in lyk2 mutants pre‐treated with elicitors. 10‐day‐old WT, lyk2‐1 and lyk2‐2 seedlings were pre‐treated with water or flg22 (10 nM) for 24 hr and subsequently treated with water or chitin (25 μg ml⁻¹) for 10 (a) or 20 min (b). Phosphorylated MPK3, MPK4, MPK6 and MPK11 were detected by immunoblot using an anti‐P44/P42 antibody. Total MPK3 and MPK6 were detected by immunoblot using anti‐MPK3 and anti‐MPK6 antibodies. Antibodies against actin were used as controls. The arrows indicate the molecular weight of marker bands (in kDa). This experiment was repeated three times with similar results