Figure 7.

Overexpression of LYK2 increases resistance to B. cinerea and expression of defence genes in responses to chitin and infection. (a) PAD3 expression in WT, 35S:LYK2 1.1 and 15.1 seedlings, treated for 3 hr with chitin at the indicated concentrations, was determined by qRT‐PCR. UBQ5 was used for normalization. Data are means (± SE, n = 3 biological replicates). (b) Four‐week‐old WT and 35S:LYK2 line 1.1 and line 15.5 seedlings were treated with water or chitin (25 μg ml⁻¹) for the indicated time. Phosphorylated MPK3, MPK4, MPK6 and MPK11 were detected by immunoblot using an anti‐P44/P42 antibody. Total MPK3 and MPK6 were detected using anti‐MPK3 and anti‐MPK6 antibodies. Antibodies against actin were used as controls. The arrows indicate the molecular weight of marker bands (in kDa). (c, d) WT and 35S:LYK2 Line 1.1 four‐week‐old plants were sprayed with water, 200 μg ml⁻¹ OG or 1 μM flg22 and inoculated after 24 hr with a B. cinerea spore suspension. PAD3 (c) and PR1 (d) expression, at the indicated times, was measured by qRT‐PCR and normalized using UBQ5. Data are means (± SE, n = 3 biological replicates). Asterisks indicate statistically significant differences between WT and 35S:LYK2 line 1.1 plants, according to Student's t‐test (*, p < .05; **, p < .01; ***, p < .001). The results are representative of three (a, c, and d) or two (b) independent experiments