FIGURE 4.

Cleaning of samples with temporary shift in signal or a monotonic decrease in signal. The top figure shows the flow rate of the sample and is followed by two scatterplots where the marker signals measured on channels V450‐a and Y780‐a are displayed. The red lines represent the medians of the marker intensities determined in bins of 1000 events per bin. At the bottom, a heatmap is shown where the events are colored whether they are removed by one of the four algorithms. The exclamation mark refers to the warning that PeacoQC gave due to the monotonic increasing or decreasing effect. (A) In this sample, the speed was set to the highest setting possible, resulting in an uptake of the sample with a lower amount of cells which influenced the signal intensity until a steady flow of events was reached. (B) A contamination was ensured in this sample by not cleaning the flow cell after measuring a DAPI stained sample. Due to the DAPI contamination into the measured sample, a monotonic increasing or decreasing effect was introduced in certain channels of the sample [Color figure can be viewed at wileyonlinelibrary.com]