FIG. 3.

Zymogram of laccase isoenzymes incubated with purified PoSl protease. Fifty microliters of 2-day-old filtered culture broth that had been treated with PMSF (1 mM) was incubated with PoSl (0.01 nmol), and aliquots of equal volumes, withdrawn at different times, were loaded onto a native PAGE gel. Lane 1, control (sample incubated for 18 h in the absence of PoSl); lanes 2 to 4, samples incubated for 1, 3 and 18 h, respectively.