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Purification of PoS1 protease
| Purification step | Vol (ml) | Total activity (U) | Total proteins (mg) | Sp act (U/mg) | Recovery (%) |
|---|---|---|---|---|---|
| Broth | 2,220 | 279 | 111 | 2.5 | 100 |
| NH4(SO4)2 precipitate | 14 | 265 | 40 | 6.6 | 95 |
| DEAE Sepharose | 2 | 226 | 2.7 | 84 | 81 |
| Superdex 75 | 1 | 84 | 0.6 | 140 | 30 |
| Mono Q | 0.5 | 36 | 0.2 | 180 | 13 |
