TABLE 3.
Effect of protease inhibitors on PoS1 activity
| Inhibitor (concn) | Residual activity (%) |
|---|---|
| HgCl2 (1 mM) | 93 |
| FeCl3 (1 mM) | 100 |
| CuSO4 (1 mM) | 77 |
| EDTA (1 mM) | 100 |
| o-Phenanthroline (1 mM) | 100 |
| p-Hydroxymercuribenzoate (1 mM) | 100 |
| p-Chloromercuribenzoate (1 mM) | 100 |
| E-64 (0.1 mM) | 93 |
| Chimostatin (0.1 mM) | 17 |
| Antipain (1 mM) | 2 |
| Pepstatin A (1 mM) | 100 |
| DCIa (0.5 mM) | 6 |
| PMSF (1 mM) | 1 |
| APMSFb (1 mM) | 6 |
| Aprotinin (1 mM) | 54 |
DCI, 3,4-dichloroisocoumarin.
APMSF, 4-amidinophenyl-methanesulfonyl fluoride.