miR combo induces cardiomyocyte gene expression in human cardiac fibroblasts.
Fetal human cardiac fibroblasts isolated from the left ventricle were transfected with miR combo or the non-targeting control miRNA negmiR. After 14 days, RNA was extracted and pooled from three separate transfections. Pooled RNA was analyzed by high-throughput sequencing. Sequences were matched to the human genome and genes significantly up- and down-regulated by miR combo were determined. Up- and down-regulated genes were analyzed via gene ontology to determine their function.
(A) Enrichment analysis displaying Gene Ontology (GO) terms which are over-represented in the set of genes up-regulated by miR combo.
(B) Enrichment analysis displaying Gene Ontology (GO) terms which are under-represented in the set of genes up-regulated by miR combo.
(C) Enrichment analysis displaying Gene Ontology (GO) terms which are over-represented in the set of genes down-regulated by miR combo.