FIG. 1.

Schematic location of the primers used and the overall PCR approach. We utilized one pair of primers, P0 and 338F, resulting in an amplicon for all eubacteria. The utilization of oligonucleotides Lm3 and P0 resulted in a PCR amplicon of about 1,400 bp specific for the genus Bifidobacterium. An amplification product with the primer pair Bflact2-Bflact5 could be achieved only for the species B. lactis.