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. 2022 Jun 8;21(7):100256. doi: 10.1016/j.mcpro.2022.100256

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 7 — Localization of targeted MicroID2 constructs. We added subcellular localization motifs to N-terminal and C-terminal domains of MicroID2 and TurboID constructs (NES, NLS, and MTS). We transiently transfected human embryonic kidney (HEK) cells with MicroID2 and TurboID constructs and performed immunofluorescence to measure subcellular localization. A, NES localization: Streptavidin-488 = biotinylation; MicroID2/TurboID expression; FLAG = MicroID2/TurboID expression. B, NLS localization: streptavidin-488 = biotinylation; FLAG = MicroID2/TurboID expression. C, MTS localization: FLAG = MicroID2/TurboID expression; TOM20 = mitochondrial outer membrane marker. D, MTS localization: Streptavidin-488 = biotinylation; TOM20 = mitochondrial outer membrane marker. A–D, the scale bar represents 20 μm. Representative of two independent experiments. MTS, mitochondrial targeting sequence; NES, nuclear export sequence; NLS, nuclear localization sequence.