Table 3.

Binding affinities of single and dual PPxY peptides to WWOX tandem domain as determined by NMR (see Experimental procedures section)

WWOX	Ligand	KD WW1 (μM)	KD WW2 (μM)
		Based on W44	Based on V73
WW1WW2	PY3	10 ± 2	>1000
WW1WW2	PY1	21 ± 2	ND
WW1WW2	PY2	15 ± 4	ND
WW1WW2	PY3PY3	0.6 ± 0.3	40 ± 20
WW1WW2	PY1PY3	6 ± 1	80 ± 20
WW1WW2	PY1PY2	10 ± 2	∼1000
WW1WW2	PY1PY2S	2 ± 1	70 ± 20

See Table 1 for details on WW domains and peptide motifs used.

Abbreviation: ND, Not Detectable.