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β-hexosaminidase release after mast cell degranulation induced by bispecific IgE antibodies. Human mast cells were sensitized with indicated mixtures of specific IgEs and AG30P unrelated IgE and added to plated (A) CHO/mPSMA cells or (B) CHO/mEGFR cells. Mean ± SD of duplicates is shown. Statistical significance was determined with two-way ANOVA with Dunnett’s multiple comparison test (p < 0.0001). CHO, Chinese hamster ovary; PSMA, prostate-specific membrane antigen.
