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. 2022 Jun 16;298(8):102153. doi: 10.1016/j.jbc.2022.102153

Figure 6.

Figure 6

Bispecific IgE induces cytotoxicity in vitro and in vivo. Real-time ADCC assay against (A) CHO/mPSMA.mEGFR cells and (B) CHO.K1 cells and hCD11b+ cells as effectors. Representative data of two independent experiments performed in duplicates are shown. Normalization was done ∼5 h after the effector cell addition. Statistical significance was determined using an extra sum-of-squares F test (p < 0.0001) (C) Rag2−/−γc−/− mice were intraperitoneally injected with CMFDA-labeled CHO/mPSMA.mEGFR target cells, indicated antibodies and hCD11b+ cells as effectors. Recovered number of live target cells (C) and hCD11b+ cells (D) after 24 h is shown. E, ratio between recovered number of live target cells and hCD11b+ cells after 24 h. Statistical significance was determined by unpaired two-tailed t test (p = 0.0044). (n = 4). CHO, Chinese hamster ovary; PSMA, prostate-specific membrane antigen.