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Dissociation of animal caps for lysosomal studies
(A) Images from a movie of animal cap excised at blastula stage 8.5 plated in fibronectin and filmed for 15 h; note that cells divide in these conditions. Scale bar, 500 μm.
(B–C′) Animal cap cells serve as a model for lysosomal studies using LAMP1-RFP or CD63-RFP as markers; the DIC visible light channel shows yolk platelets present in the embryonic cells. See also Methods video S1. Scale bar, 10 μm.
