REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Digitonin	Sigma	Cat#300410
Sir-Lysosome	Cytoskeleton Inc	Cat#CYSC012
Tetramethylrhodamine Dextran (TMR-Dx) 70,000 kDa	Thermo Fisher Scientific	Cat# D1818
Lithium chloride (LiCl)	Sigma	Cat#L4408
10-cm dish	Thermo Fisher Scientific	Cat#174903
8-well glass-bottom chamber slides	ibidi	Cat#80827
Circular coverslips	ibidi	Cat#10815
Culture chambers #0 cover glass	E&G	Cat#GBD00003-200
Leibovitz L-15 cell culture medium	Thermo Fisher Scientific	Cat#11415064
Glutamine	Thermo Fisher Scientific	Cat#25030081
Bovine Serum Albumin (BSA)	Thermo Fisher Scientific	Cat#9048468
Pen-Strep antibiotics	Thermo Fisher Scientific	Cat#15140122
Triton X-100	Thermo Fisher Scientific	Cat#HFH10
Paraformaldehyde (PFA)	Sigma	Cat#P6148
Fibronectin	Sigma	Cat# F4759
PBS for cell culture applications	Gibco	Cat#10-010-023
PBS for immunostaining washes	Thermo Fisher Scientific	Cat#BP3994
Mounting Medium with DAPI	Abcam	Cat# ab104135
Protease Inhibitors	Roche	Cat#04693132001
Phosphatase inhibitors	Calbiochem	Cat#524629
mMESSAGE mMACHINE™ SP6 Transcription Kit	Thermo Fisher Scientific	Cat#AM1340
Experimental models: Organisms/strains
Xenopus laevis (wild-type)	Xenopus I	N/A
Recombinant DNA
LifeAct	IMSR	RRID: IMSR_EM:12427
pCS2-mGFP	Addgene	RRID: Addgene_14757
CD63-RFP	Addgene	RRID: Addgene_62964
xWnt8myc	Addgene	RRID: Addgene_16863
Software and algorithms
ImageJ	NIH	http://imagej.nih.gov/ij/
Axiovision 4.8	ZEISS	http://Zeiss.com
Zen 2.3 imaging software	ZEISS	http://Zeiss.com
Other
IM 300 microinjection pump	Narishige International USA, Inc	N/A
Axio Observer Z1 Inverted Microscope with Apotome	ZEISS	N/A