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. 2022 Jul 13;3(3):101532. doi: 10.1016/j.xpro.2022.101532

Figure 5.

Figure 5

Micro-manager software and selection of the arena area-of-interest for sleep detection

Using the rectangle tool within ImageJ, drag a box across the entire arena, record the dimensional parameters, x and y position (of top left corner) and width (w) and height (h), and enter them into the script code.