Figure 7.

Example behavioral and neural data

(A) Raster plot of sleep events (black) over 12 h, sorted by total sleep fraction (n = 535 animals, Lawler et al., 2021). Note that these data were obtained using a large population microfluidic device, not described in this protocol.

(B) Example single animal behavior in a typical 10-h closed-loop experiment indicating automatically triggered neural recording trials. Sleep and awake states are indicated by black and gray shading. Recordings were triggered one minute after a sleep state change, followed by 15 min of rest (white shading).

(C) Heatmap of individual ASH neural responses to 10 s stimulation with 1 mM CuCl₂ (gray bar), in sleeping and awake animals.

(D) Average ASH neural responses in sleep and awake states from panel C (n = 18 sleep, 17 awake). Shading represents s.e.m. No change is observed in ASH sensory responses between the sleep and wake states, indicating a consistent sensory response. Other neurons may change in peak magnitude, duration, rise time, decay rate, or other metric across sleep states.