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Saline buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 80 mM | 4.68 g |
| KCl | 5 mM | 0.373 g |
| D-glucose | 20 mM | 3.60 g |
| HEPES | 10 mM | 2.38 g |
| MgCl2 | 5 mM | 1.02 g |
| CaCl2 | 1 mM | 0.111 g |
| ddH2O | n/a | to 1 L |
| pH to 7.2 | n/a | n/a |
| Total | n/a | 1 L |
Store at room temperature (15°C–25°C) for up to 1 year.
