Nematode Growth Medium Agar

Reagent	Final concentration	Amount
NaCl	50 mM	3 g
Agar	50 mM	17 g
Peptone	10 mM	2.5 g
5 mg/mL cholesterol in ethanol	∼13 mM	1 mL
1 M KPO4 buffer pH 6.0	25 mM	25 mL
1 M MgSO4	1 mM	1 mL
1 M CaCl2	1 mM	1 mL
ddH2O	n/a	975 mL
Total	n/a	∼1 L

Mix dry ingredients and water and autoclave on liquid cycle. When cooled to 55°C, add the four liquid additives, mix, and pour into Petri dish plates under sterile conditions. Store plates at room temperature (15°C–25°C) for several weeks or at 4°C for several months.