REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli (OP50)	Caenorhabditis Genetics Center (CGC)	OP50
Experimental models: Organisms/strains
Young adult C. elegans hermaphrodites expressing GCaMP in desired neurons	Caenorhabditis Genetics Center (CGC) or corresponding authors of published work	N/A
C. elegans ASH::GCaMP3 strain	Larsch et al. (2013)	CX10979
Chemicals, peptides, and recombinant proteins
HEPES	Sigma-Aldrich	Cat# H3375
Magnesium chloride, MgCl2	Sigma-Aldrich	Cat# M2393
Calcium chloride, CaCl2	Sigma-Aldrich	Cat# C3881
Sodium chloride, NaCl	Sigma-Aldrich	Cat# S7653
Potassium chloride, KCl	Sigma-Aldrich	Cat# P3911
Copper chloride, CuCl2	Sigma-Aldrich	Cat# 751944
D-glucose	Sigma-Aldrich	Cat# G7021
Nematode Growth Medium (NGM) agar	Stiernagle (2006)	N/A
Poly(dimethyl siloxane) (PDMS)	Ellsworth Adhesives	Sylgard 184
(tridecafluoro-1,1,2,2-tetrahydrooctyl)trichlorosilane (TFOCS)	Gelest	CAS# 78560-45-9
Fluorescein, Sodium salt	Sigma-Aldrich	Cat# F6377
Software and algorithms
Custom control code: ClosedLoopStimulation	Lawler et al. (2021) and this work	https://albrechtlab.github.io https://github.com/albrechtLab/ClosedLoopStimulation 10.5281/zenodo.6629830
Custom analysis code: Neurotracker	Larsch et al. (2013)	https://github.com/albrechtLab/Neurotracker 10.5281/zenodo.6632736
Micro-manager	Micro-manager	https://micro-manager.org/
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
Arduino IDE	Arduino	https://www.arduino.cc/en/software
ImageJ	Schneider et al. (2012)	https://imagej.nih.gov/ij/
Other
ValveLink 8.2 digital/manual valve controller	AutoMate Scientific	Cat #01-18
Arduino nano	Arduino	Cat #A000005
Wires and connectors	Various	See Figure 2B
3-way miniature inert PTFE isolation valve	Parker	Cat #001-0017-900
Pinch valve, normally open	NResearch	Cat #161P021
SOLA light engine (fluorescence)	Lumencor	Cat #SM6-LCR-SA
Axio Observer.A1 inverted microscope set up for epifluorescence (GFP filter cubes, 5× objective or similar)	Zeiss	Cat #491237-0012-000
Illuminator microLED (brightfield)	Zeiss	Cat #423053-9072
Orca Flash 4.0 Digital sCMOS camera	Hamamatsu	Cat #C11440-22CU
Optogenetic stimulation LED and controller (optional)	Mightex	Cat #PLS-0625-030-S and #SLA-1200-2
Petri dishes (60 mm)	Tritech	Cat #T3305
Glass slide, 1 mm thick	VWR	Cat #75799-268
Glass scribe	Ted Pella	Cat #54468
Diamond drill bit	Dremel	Cat #7134
Microfluidic device	Larsch et al. (2013); Lagoy and Albrecht (2015)	N/A
Microfluidic tubing, 0.02″ ID	Cole-Parmer	Cat #EW-06419-01
Luer 3-way stopcock	Cole-Parmer	Cat #EW-30600-07
Luer 23 ga. blunt needle	VWR	Cat #89134-100
Tube 19 ga., 0.5″	New England Small Tube	Cat #NE-1027-12
Microfluidic device clamp	Warner Instruments (or machine shop)	P-2