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. 2022 Feb 16;12(7):3124–3138. doi: 10.1016/j.apsb.2022.02.008

Figure 6.

Fig. 6

PEG-AuNPs block autophagic flux and cause lysosomal dysfunction in macrophages. (A) Western blotting analysis of autophagy-related proteins. (B) The images of representative immunofluorescence and quantitative analysis of the number of yellow autophagosomes (G+R+) and red autolysosomes (G−R+) of mRFP-GFP-LC3 dots in macrophages. Scale bar = 10 μmol/L. (C) Fluorescence images of LysoSensor Green DND-189 stained macrophages. Scale bar = 50 μmol/L. (D) Flow cytometry analysis of LysoSensor Green DND-189 stained macrophages. (E) Flow cytometry analysis of macrophages stained with acridine orange (AO). Data are expressed as mean ± SD (n = 5). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.