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. 2021 Aug 26;9(5):1357–1367. doi: 10.1016/j.gendis.2021.08.005

Figure 2.

Figure 2

The loss of RGS12 in macrophages attenuates the cartilage destruction and inflammation in OA mouse models. (A) Safranin O staining of the cartilage from WT and RGS12 cKO groups at 4 weeks post-surgery. (B,C) The safranin O positive area and cartilage OARIS scores were analyzed as depicted in (A). The data in the figures represent the mean ± SEM. Significant differences between WT and RGS12 cKO groups are indicated as ∗∗P < 0.01, ∗∗∗P < 0.001, vs. WT group, n = 5. (D) The ubiquitin (Ub) levels were detected by immunoblotting in synovial tissues lysis from (A). Quantitative analysis of Ub level was depicted on the right. The data are represented as means ± SEM. ∗P < 0.05, n = 3. (E) Western blot analysis of the levels of IL1β, IL6, and TNFα in the synovial tissues from (A). β-Actin was used as a loading control. (F) Quantitative analysis of the Western blots in Figure 1E. The data are represented as means ± SEM. ∗∗∗P < 0.001, n = 3.