Table 1.
Composition and nutrient levels of basal diet (dry matter basis, g/kg).
| Item | Content |
|---|---|
| Ingredients | |
| Casein | 135.0 |
| Gelatin | 44.7 |
| Soybean protein concentrate | 220.0 |
| α-starch | 240.0 |
| Corn starch | 209.1 |
| linseed oil | 17.7 |
| Soy oil | 14.5 |
| Cellulose | 50.0 |
| Ca(H2PO4)2 | 14.8 |
| Vitamin premix 1 | 10.0 |
| Mineral premix 2 | 20.0 |
| Vitamin D3 (VD3) premix 3 | 10.0 |
| Choline chloride (50%) | 10.0 |
| Ethoxyquin (30%) | 0.5 |
| DL-Met | 3.7 |
| Total | 1,000.0 |
| Nutrients content4 | |
| Crude protein | 300.0 |
| Crude fat | 37.8 |
| n-3 PUFAs | 10.4 |
| n-6 PUFAs | 9.6 |
| n-3: n-6 PUFAs | 10.8 |
| Available phosphorus | 4.0 |
| VD3, μg/kg | 3.8 |
PUFAs = polyunsaturated fatty acids.
One kilogram of vitamin premix contained the following (g/kg): DL-α-tocopherol acetate (50%), 12.58; menadione (22.9%), 0.83; cyanocobalamin (1%), 0.94; D-biotin (2%), 0.75; folic acid (95%), 0.42; thiamine nitrate (98%), 0.09; ascorbyl acetate (95%), 4.31; niacin (99%), 4.04; meso-inositol (98%), 19.39; calcium-D-pantothenate (98%), 3.85; riboflavin (80%), 0.73; pyridoxine hydrochloride (98%), 0.62; retinyl acetate (500,000 IU/g), 2.10. All ingredients were diluted with maize starch to 1 kg.
One kilogram of mineral premix contained the following (g/kg): FeSO4.H2O (30.0% Fe), 12.2500; MgSO4.H2O (15.0% Mg), 200.0000; ZnSO4.H 2O (34.5% Zn), 8.2460; MnSO4.H2O (31.8% Mn), 2.6590; CuSO4.5H2O (25.0% Cu), 0.9560; Na2SeO3 (44.7% Se), 0.0168; KI (76.9% I), 0.0650g. All ingredients were diluted with maize starch to 1 kg.
VD3 premix: premix was added to obtain graded level of VD3 and the amount of maize starch was reduced to compensate.
The contents of crude protein and crude lipid were measured values on the air-dried matter basis. Supplement of available P, n-3 PUFAs, and n-6 PUFAs were calculated according to NRC (2011).