Table 2.
Real-time PCR primer sequences.
| Gene | Forward sequences of primers (5′ to 3′) | Reverse sequences of primers (5′ to 3′) | Temperature, °C | GenBank number |
|---|---|---|---|---|
| SLC7A7 | GATAGCCATCACTTTCTCCAAC | GTCCCATACTTCACATAGGCA | 58.0 | JX013494 |
| SLC7A6 | GCAGAGTTGGGCACGACAATCAC | GGAAGAGCGGCTGAACCAAGTAG | 58.0 | KC206055 |
| SLC1A2a | ATCCATGGTGCCATATTCCTG | CAACGGAAAGTTACAGGCAGAG | 59.6 | KY200977 |
| SLC1A3 | GGAATGCTTTCGTCATCCTCAC | CAGAGCGGCCATACCTGTAATT | 61.0 | KY200978 |
| SLC1A5 | ACACTTTCTTGCTGGGATTGGT | GGATGGTGATGACCTGGACAAA | 62.0 | KU559898 |
| SLC6A14 | TCATTGCGTACCCTGATGCTCT | ACTTCAGAACTTTGGGATAGGCA | 62.5 | KX823959 |
| SLC6A19b | ACCACTGGAAAGGCTGTTTATGT | ACCGCATCTTGCTCACAGTTATT | 62.0 | KX823960 |
| SLC7A1 | CGACATCACTGAACCCTCCAAC | TGCACAGGCTGAACAGGACACT | 62.0 | KY200979 |
| SLC7A5 | CAACATGAGCCGACCAGGAGAC | CCAGCGACAACCCGACTGAACC | 62.0 | KY200980 |
| SLC7A8 | TGGTGAGAAGCTGTTGGGAGTGA | GCAAGTGAAGAGTAGGGCTGGAA | 59.6 | GU474428 |
| SLC7A9 | TTCTACAGTCTTCTGCCGTTGC | AGAGCTGGAGAAGGCGTGTAAC | 61.5 | KX823958 |
| SLC38A2 | AGAAGAGTCCTGCAAACCCAA | CACAAACATTCCCAGAAACGA | 61.7 | KY200981 |
| SLC6A6 | CTCCGCAAGAACAAGACACTC | CCCACACAGCGAGCAGAC | 62.5 | KX682391 |
| SLC15A1 | TGCTCTTGTTGTGTTCATCG | CTCTCTCTTGGGGTATTGCTT | 62.0 | KC782748 |
| atp1a1a.1 | TGCCATTGTAGCCGTAAC | GGTGCCCAAAGGTAGAGG | 60.3 | JX854442 |
| atp1a1a.4 | GAGGTCGTTGCTGGTGAT | CAGTGAGGGAAGAGTTGTC | 55.9 | KM112094 |
| CK | CTCCTCGTTCACCCAGAC | CAGCATCAAGGGATACGC | 61.4 | JX854444 |
| TOR | TCCCACTTTCCACCAACT | ACACCTCCACCTTCTCCA | 61.4 | JX854449 |
| S6K1 | TGGAGGAGGTAATGGACG | ACATAAAGCAGCCTGACG | 59.4 | EF373673.1 |
| 4E-BP1 | GCTGGCTGAGTTTGTGGTTG | CGAGTCGTGCTAAAAAGGGTC | 60.3 | KT757305.1 |
| ATF4 | CAACATTAGCAGCGGCAA | GTGAGCGGGTGAACGGT | 56.3 | AY437846.1 |
| β-actin | GGCTGTGCTGTCCCTGTA | GGGCATAACCCTCGTAGAT | 61.4 | M25013 |
SLC = solute carrier; atp1a1a.1 = Na+/K+-ATPase alpha-subunit isoform 1; atp1a1a.4 = Na+/K+-ATPase alpha-subunit isoform 4; CK = creatine kinase; TOR = target of rapamycin; S6K1 = ribosomal protein S6 kinase 1; 4E-BP1 = eIF4E-binding protein 1; ATF4 = activating transcription factor.