Table 1.

Primer sets for quantitative and qualitative RT-PCR analysis of gene expression

Gene	Primers
Human ABCC4
Forward	5′-AGTCAATTCTGAAAGCTCCGGTA-3′
Reverse	5′-CGGCAGCAAATCATCCAAGTG-3′
Human HIPK3
Forward	5′-GCACATGTTGTCTGGCCTCAG-3′
Reverse	5′-TCTCTTCCTGGCTCCCATTCC-3′
Human circHIPK3 [divergent]
Forward	5′-TATGTTGGTGGATCCTGTTCGGCA-3′
Reverse	5′-TGGTGGGTAGACCAAGACTTGTGA-3′
Human circHIPK3 [convergent]
Forward	5′-TTAGTCCCCTGCCACTAAAAGTGA-3′
Reverse	5′-TGCCGAACAGGATCCACCAACATA-3′
Human β-ACTIN [divergent]
Forward	5′-CACCTTCACCGTTCCAGTTT-3′
Reverse	5′-AGTCATTCCAAATATGAGATGCG-3′
Human β-ACTIN [convergent]
Forward	5′-AAACTGGAACGGTGAAGGTG-3′
Reverse	5′-CGCATCTCATATTTGGAATGACT-3′
Human pre-circHIPK3
Forward	5′-TATTTGCACTGCCAACTAGTTAAG-3′
Reverse (qRT-PCR)	5′-CTGAATCCTTATACAGGTTGAGCA-3′
Reverse (direct sequencing)	5′-CTAAAGCGCATGCTCCAGAC-3′
Human 18S rRNA
Forward	5′-AGAAGCCCCTGGCACTCTAT-3′
Reverse	5′-GCAAAGTGGGCACAGTGATG-3′
Luciferase
Forward	5′-CAGCGAGAATAGCTTGCAGT-3′
Reverse	5′-AGGATCTTTTGCAGCCCTTT-3′
Renilla Luciferase
Forward	5′-GAGAAGGGCGAGGTTAGACG-3′
Reverse	5′-CCCGAAGGTAGGCGTTGTAG-3′