Figure 10.

Uptake of pyridoxine and thiamine in transiently transfected HEK293 cells expressing the SLC19A3s of selected animal species.A, the uptake of [³H]pyridoxine (5 nM) was evaluated for 2 min at pH 5.5 and 37 °C in cells expressing the SLC19A3s of selected animal species. B, the uptake of [³H]thiamine (5 nM) was similarly evaluated for 2 min at pH 7.4 and 37 °C. The uptake of pyridoxine and thiamine in mock cells as a control was 11.0 and 8.5 fmol/min/mg protein, respectively. No transporters had the EGFP tag. Data are presented as means ± SD (n = 3). ∗p < 0.05 compared with control. EGFP, enhanced GFP; HEK293, human embryonic kidney 293 cell line; SLC19A3, solute carrier SLC19A3.