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. 2001 Jun;67(6):2775–2780. doi: 10.1128/AEM.67.6.2775-2780.2001

TABLE 1.

Sampling and endotoxin extraction methods for sawmills and swine barns

Type of sampling and location Sampling method Flow rate (liters/min) Mean vol of air sampled (liters) Extractiona
Container Method
Impinger sampling of sawmills and swine barns AGI, 20.0 ml of pyrogen-free saline 12.5 200 Polypropylene tube Vortex mixer, 10 min, 20°C
Filter sampling
 Sawmills 0.8-μm-pore-size PVCb filter, IOM inhalable dust cassette 2.0 862 ± 158 Polypropylene tube Sonication bath, 1 h, 20°C
 Swine barns 0.8-μm pore-size PVC filter, closed-face cassette 1.5 361 ± 3 Borosilicate glass jar Shaking bath, 16 h, 37°C
a

The extraction solution was pyrogen-free water with 0.04% Tween 80 in each case. 

b

PVC, polyvinyl chloride.