TABLE 1.
Sampling and endotoxin extraction methods for sawmills and swine barns
Type of sampling and location | Sampling method | Flow rate (liters/min) | Mean vol of air sampled (liters) | Extractiona
|
|
---|---|---|---|---|---|
Container | Method | ||||
Impinger sampling of sawmills and swine barns | AGI, 20.0 ml of pyrogen-free saline | 12.5 | 200 | Polypropylene tube | Vortex mixer, 10 min, 20°C |
Filter sampling | |||||
Sawmills | 0.8-μm-pore-size PVCb filter, IOM inhalable dust cassette | 2.0 | 862 ± 158 | Polypropylene tube | Sonication bath, 1 h, 20°C |
Swine barns | 0.8-μm pore-size PVC filter, closed-face cassette | 1.5 | 361 ± 3 | Borosilicate glass jar | Shaking bath, 16 h, 37°C |
The extraction solution was pyrogen-free water with 0.04% Tween 80 in each case.
PVC, polyvinyl chloride.