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. 2022 Apr 20;30(4):1431–1443. doi: 10.1007/s10787-022-00967-3

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© The Author(s) 2022

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Fig. 1 — Expression of Nrf2 and Keap-1 in the alveolar macrophages of NS, S and COPD patients. Protein expression of Nrf2 and Keap-1 was examined by immunohistochemistry in lung tissue from NS (n = 12), S (n = 12) and COPD (n = 12). Data presented as percentage of positive cells of alveolar macrophages (AM) for Nrf2 (A) and Keap-1 (B). The number of alveolar macrophages per area of airways in lung tissue was determined (C). mRNA expression levels of NFE2L2 (D) and KEAP1 (E) were determined by RT-qPCR in AM from NS (n = 8), S (n = 25) and COPD (n = 29). Data represent individual patients with mean. RT-qPCR data expression relative to an endogenous control (2^−ΔC_t)