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. 2022 Jul 18;12:12231. doi: 10.1038/s41598-022-16304-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Schematic of the magnetically aligned lipid bilayer (blue) and inserted Pf3_24_2 (gray). θ is the angle formed between the helix long axis of Pf3_24_2 and the membrane normal. (b) ¹H-¹⁵N SAMPI4 spectra of Pf3_24_2 (Fig. 1d) in the DMPC/DHPC bicelles ([DMPC]/[DHPC] = 3) aligned with their normal perpendicular to the magnetic field. The simulated PISA wheels with varied tilt angles θ (0–70°) are overlaid onto the spectra. The bicelle order parameter S_mol is fixed at 0.9. (c) ¹H-¹⁵N SAMPI4 spectra of Pf3_24_2 in the absence (blue) and presence (red) of 2 mol% of mini-MPIase-3.