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. 2022 Jul 18;12:12258. doi: 10.1038/s41598-022-16506-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SIRT5OE mice exhibit decreased mildly decreased cardiac lysine malonylation. (A) Immunoblot analysis of SIRT5 expression in heart samples presented in c-e. Original immunoblot images are presented in Supplemental Fig. 7A. (B) Weight (g) of WT and SIRT5OE male and female mice with age. (C-E) Representative immunoblot analysis of succinyl-lysine (Ksucc), and malonyl-lysine (Kmal) levels in WT and SIRTOE hearts, with quantification. Original immunoblot images are presented in Supplemental Fig. 7B-C.