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. 2022 Jul 18;12:12258. doi: 10.1038/s41598-022-16506-7

Figure 2.

Figure 2

SIRT5OE mice are protected against TAC-induced heart failure. Echocardiography was performed on WT sham (n = 16), WT TAC (n = 12), SIRT5OE sham (n = 10) and SIRT5OE TAC (n = 14) mice to measure changes in cardiac function four weeks post-surgery. (A) Depiction of groups, procedures, and timeline of surgery. (B) Aortic pressure gradient in mice after TAC. Echo measurements for (C) systolic interventricular septum (IVS) thickness; (D) systolic posterior wall thickness (PWT); (E) LV end-diastolic diameter; (F) left ventricle mass normalized to body weight; (G) fractional shortening; (H) ejection fraction. (I) Quantification of CM cell area, normalized to WT sham four weeks after surgery [WT sham (n = 4), WT TAC (n = 4), SIRT5OE sham (n = 4) and SIRT5OE TAC (n = 10)]. (J) Representative wheat germ agglutinin-stained cardiac sections of the indicated genotypes and treatments four weeks post-surgery. Scale bar = 50 um. (K) qRT-PCR for Acta1 (n = 3 for all groups); Nppa and Myh6 [WT sham (n = 5), WT TAC (n = 5), SIRT5OE sham (n = 3) and SIRT5OE TAC (n = 6)]; and Myh7 [WT sham (n = 5), WT TAC (n = 4), SIRT5OE sham (n = 3) and SIRT5OE TAC (n = 5)] expression normalized to GAPDH. Statistical significance was determined using Student’s t-test for 2-group analysis or two-way ANOVA followed by Sidak’s correction for multiple comparisons for 4-group analyses.