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. 2022 Jul 18;12:12258. doi: 10.1038/s41598-022-16506-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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WT TAC mice exhibit a metabolic shift by RNA-seq, but are functionally comparable to SIRT5OE TAC mice four weeks post-surgery. (A) GSEA of SIRT5OE TAC compared to WT TAC RNA-seq samples. NES: normalized enrichment score. FDR: false discovery rate. (B-C) Immunoblot analysis of electron transport chain subunits and quantification of CV-ATP5A. Original immunoblot images are presented in Supplemental Fig. 8A. (D) Agilent/Seahorse analysis of electron flow through the ETC in mitochondria isolated from WT (n = 5) and SIRT5OE (n = 6) hearts 4 weeks after TAC. y-axis indicates oxygen consumption rate (OCR) in pmol/min. (E) qPCR of mitochondrially encoded (mtDNA) genes 16S and ND1, normalized to HK2, encoded by the nuclear genome (nuDNA). (F) Sod2 mRNA levels measured by RNA-seq. (G-H) SOD2 immunoblot from heart lysates and quantification. Original immunoblot images are presented in Supplemental Fig. 9A.