Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 18;12:12268. doi: 10.1038/s41598-022-16411-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Reactivity of monoclonal and polyclonal antibodies against clinically relevant mutated variants of domain III of HER-1 conferring cetuximab treatment resistance. Recognition of phage-displayed domain III variants by monoclonal antibodies (a) and pooled polyclonal purified antibodies from HER-1 immunized mice (b) was evaluated through phage ELISA. The original phage-displayed HER-1 domain III and its mutated variants were incubated on microtiter plates coated with the different antibodies. Bound phages were detected with an anti-M13 mAb labeled with horseradish peroxidase. hT1 mAb and immunoglobulins from non-immunized mice (control IgG) were used as negative controls.