Table 1.

Display levels of the individual HER domains.

Antigen	Domain	Display levels (AU/mL)
HER-1	I	367.5 ± 4.8
	II	28.9 ± 3
	III	159.9 ± 12.8
	IV	49.8 ± 18.2
HER-2	I	273.9 ± 17.8
	II	105.8 ± 5.4
	III	245.2 ± 2.0
	IV	71.4 ± 14.3

Phages displaying every individual HER domain were rescued with M13KO7 helper phage and purified through polyethylene glycol precipitation. Each phage preparation was diluted and titrated on polyvinyl chloride microplates coated with the anti-c-myc tag antibody 9E10. Bound phages were detected with an anti-M13 antibody conjugated to horseradish peroxidase. The standard curve of phages displaying c-myc only with no fused protein was simultaneously evaluated and used to calculate the relative display levels for each preparation. A display level of 100 arbitrary units (AU)/mL was assumed for the undiluted standard. Numbers indicate the mean value ± standard deviation of three independent phage preparations displaying each domain.