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. 2022 Jul 18;9:418. doi: 10.1038/s41597-022-01470-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Overview of experimental design and study workflow. (a) Reversal of ethanol-induced apoptosis in mouse primary liver cells in νitro served as a model for this microarray study. Phase-contrast images of (i) untreated cells, (ii) cells undergoing apoptosis triggered by 4.5% ethanol (EtOH) for 5 hours, and (iii) ethanol-treated cells washed with and then incubated in fresh medium for 24 hours. (i’-iii’) Merged confocal images, with conditions like i-iii, for visualizing mitochondria (red), nuclei (blue), and cell morphology (DIC). (b) An outline of the workflow for the time-course microarray study. Mouse primary liver cells were treated with 4.5% ethanol for 5 hours to induce apoptosis (R0), and then were washed with and further incubated in fresh culture medium for 3 hours (R3), 6 hours (R6), 24 hours (R24), and 48 hours (R48) to allow reversal of apoptosis. Untreated cells served as control (Ctrl). Cell samples of three biological replicates from each condition mentioned above were collected for RNA extraction, analysed using Illumina Mouse WG-6 v2.0 Expression BeadChip for whole-genome expression profiling, and performed quality check by Partek Genomics Suite (GS) v6.6.