Fig. 8.

IFN-γ and granzyme B expression in hypoxia-treated PBMC-derived NK cells. Freshly isolated NK cells (a, b) or IL-2-expanded NK cells (c, d) or antibody-expanded NK cells (e, f) were pre-incubated at 21% O₂ or 1% O₂ in the presence of IL-2 for 72 h before being transferred to plates coated with ULBP-1 or anti-NKp46 or anti-CD16 antibodies and incubations continued under the same conditions for another 18 h. After incubation, supernatant was collected, and ELISA performed in duplicates to test the concentrations of IFN-γ and granzyme B. Results are presented as mean of three different donors. Statistical significance was analyzed between 21% O₂ and 1% O₂ by two-tailed paired Student’s t-test