Fig. 7.

S100A8 inhibits inflammation and oxidative stress. ELISA of the serum levels of TNF-α (A) and IL-6 (B). In rats treated with transfected ADSCs, the serum levels of oxidative stress-related factors MDA (C) and SOD (D) were detected by ELISA. As markers of wound healing, the expression of VEGF (E) and CTGF (F) was measured by ELISA. The expression of VEGF, CTGF, S100A8, NF-κB, phosphorylated NF-κB, IκBα, phosphorylated IκBα in healing tissue were detected by Western blot (G) and quantified (H),1: FSW, 2: FSW + ADSCs (op S100A8), 3: FSW + ADSCs (op CTL), 4: FSW + ADSCs (sh S100A8), 5: FSW + ADSCs (sh CTL). N = 3 animals/condition; ∗P < 0.05; ∗∗P < 0.01.