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. 2001 Jun;67(6):2799–2809. doi: 10.1128/AEM.67.6.2799-2809.2001

TABLE 1.

Units for expression of enumerations of attached and unattached subsurface microorganisms

Medium Expression Calculationa
Groundwater Per ml or cm3 of groundwater Default
Groundwater Per cm3 of in situ porous medium (Cells/ml) · n
Particulate solids Per g dry weight of solids (Cells/g [wet wt]) · (1 + %M)
Particulate solids Per cm3 of in situ porous medium (Cells/g [dry wt]) · (bulk density, g/cm3)
Particulate solids Per cm3 of solids (Cells/g [dry wt]) · (specific gravity, g/cm3)
Particulate solids Per cm3 of equivalent pore volume (Cells/g [dry wt]) · (g [dry wt]/ml of pore water)
Particulate solids Per cm2 of external surface area (Cells/g [dry wt]) · (g [dry wt]/SAext)
Particulate solids Per cm2 of total surface area (Cells/g [dry wt]) · (g [dry wt]/SAtot)
Particulate solids Per cm2 of adjusted surface area (Cells/g [dry wt]) · (g [dry wt]/SAadj)
Fracture face Per cm2 of external surface area (Cells/ml of extract) · (ml of extract/SAfract)
a

Cells is a generic microbiological enumeration or other quantification, e.g., biomass; n is porosity; %M is percent moisture; SAext is the external surface area of particles; SAtot is the total surface area of particles; SAadj is the adjusted surface area of particles, which includes the external and internal surfaces accessible to bacteria; SAfract is the external surface area of fracture surfaces; ml of extract is the volume of fluid in which organisms removed from fracture faces are suspended.