Figure 1.

Effects of IgA antibodies on IgE mediated degranulation of bone marrow derived mast cells. Flow cytometry plots from a representative experiment (left) and aggregate data (n=3) bar plots (right) of percent LAMP-1 expression of IgE sensitized BMMCs following antigen exposure. BMMCs were sensitized with anti-TNP IgE (αTNP IgE) (50 ng/ml). Subsequently, some cells were co-incubated with anti-TNP IgA (αTNP IgA) (100 µg/ml) (purified from TIB-194 hybridoma) or anti-OVA IgA [αOVA IgA (100 µg/ml)]. Cells were primed with antibodies overnight, then washed and stimulated with, 50 ng/ml of TNP-BSA for 10 minutes before assessing activation by staining with anti-mouse c-Kit, and LAMP-1. Statistical analysis done by ANOVA. Data shown mean ± SEM of one experiment representative of three independent experiments. ****P < .0001.