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. 2022 Jul 5;13:881655. doi: 10.3389/fimmu.2022.881655

Figure 4.

Figure 4

Effect of IgA antibodies on IgE-induced phosphorylation of Syk and on cytokine production by activated bone marrow derived mast cells (A) IgA effects on Syk phosphorylation. IgE sensitized BMMCs were incubated with or without anti-TNP IgA and stimulated with antigen for up to two minutes followed by measurement of phosphorylated-Syk (phospho-Syk) using flow cytometry (upper panel). Representative Syk phosphorylation blots and compiled ratios of phospho protein/total protein intensities in anti-TNP IgE-sensitized BMMCs or anti-TNP IgE/IgA treated BMMCs (lower panel). This experiment is representative of three replicates. (B) IgA effects on cytokine production. Cytokine (IL-6, IL-13 and TNF-α) levels in the supernatants of anti-TNP IgE sensitized BMMC incubated with or without anti-TNP IgA and stimulated with TNP-BSA for 6 hours. Statistical analysis done by ANOVA. Data shown mean ± SEM of one experiment representative of three independent experiments. ***P < .001.