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. 2022 Jul 5;13:881655. doi: 10.3389/fimmu.2022.881655

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Copyright © 2022 El Ansari, Kanagaratham, Burton, Santos, Hollister, Lewis, Renz and Oettgen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of peanut-specific IgA on basophils from peanut allergic subjects (A) IgA effects on allergen induced degranulation. Dose response analysis of effects of IgA from peanut allergic subjects on peanut induced activation of basophils from a peanut allergic donor. Data shown from one experiment run in triplicate. Statistical analysis by ANOVA. ***P < .001, ****P < .0001 (B) IgA binding to basophils. Representative flow cytometry plots showing human IgA purified from post-OIT sera labeled with FITC binding to T cells, neutrophils and basophils from whole blood. T cells were identified by the presence of CD3, neutrophils by CD45, CD14, CD16, and basophils by CCR3 and FcεRI.