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. 2022 Jul 5;9:695004. doi: 10.3389/fcvm.2022.695004

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Copyright © 2022 Chen, Liu, Cao, Hu, Wang, Wu, Liu and Yiu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Paeonol increased the MMP and viability, while decreasing the apoptosis and ROS, and suppressing miR-21-5p expression in the DOX-induced cardiomyocytes. (A) The viability of H9c2 cells treated with DOX or paeonol was detected by CCK-8 assay. (B,C) The apoptosis of H9c2 cells treated with DOX or paeonol was determined via flow cytometry. (D,F) The level of ROS in the H9c2 cells treated with DOX or paeonol was visualized by H2DCFDA staining. (E,G) The MMP in the H9c2 cells received the treatment with DOX or paeonol and was detected using TMRM staining. (H) The expression of miR-21-5p in H9c2 cells treated with DOX or paeonol was quantified with the help of RT-qPCR. U6 served as an internal control. ^∧P < 0.05, ^∧∧∧P < 0.001 vs. Control; **P < 0.01, ***P < 0.001 vs. DOX. DOX, doxorubicin; MMP, mitochondrial membrane potential; ROS, reactive oxygen species.