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. 2022 Jul 5;9:695004. doi: 10.3389/fcvm.2022.695004

FIGURE 7.

FIGURE 7

(A) The expression of SKP2 in the miR-21-5p-treated DOX-induced H9c2 cells was calculated using RT-qPCR. GAPDH was used as an internal control. (B,C) The expression of SKP2 in the miR-21-5p-treated DOX-induced H9c2 cells was detected by western blot, where GAPDH was used as an internal control. (D) The levle of SKP2 in the miR-21-5p-treated DOX-induced H9c2 cells was calculated using RT-qPCR. GAPDH was used as an internal control. (E) The viability of the miR-21-5p-treated DOX-induced H9c2 cells was evaluated based on CCK-8 assays. (F,G) The apoptosis of the miR-21-5p-treated DOX-induced H9c2 cells was determined by flow cytometry. (H,I) The ROS of the miR-21-5p-treated DOX-induced H9c2 cells was observable following the H2DCFDA staining (Magnification: ’200). (ΔP < 0.05, ΔΔΔP < 0.001 vs. Control; *P < 0.05, ***P < 0.001 vs. DOX+shNC; ###P < 0.001, vs. DOX+Paeonol+shNC; +P < 0.05, ++P < 0.01 vs. DOX; ∧∧∧P < 0.001, vs. DOX+M+NC). (DOX, doxorubicin; ROS, reactive oxygen species; M, miR-21-5p mimic; NC, negative control).