FIGURE 4.

Effect of AM on intracellular cGMP levels measured by ELISA in untreated (basal) and TNF-α-stimulated C- and GD-HUVECs after preincubation for 24 h with AM. Ionomycin (2 μM, 24 h) was used as a positive control. l-NAME (1 mmol/L) was added 45 min before AM and Ionomycin. Data are expressed as pmol/well and results by mean ± SEM, n = 4. ANOVA and Tukey’s multiple comparison test: *p < 0.05 TNF-α vs. basal; TNF-α+AM vs. TNF-α; TNF-α +AM+L-NAME vs. TNF-α+AM; **p < 0.01 IONO vs. basal; ***p < 0.001 IONO+L-NAME vs. IONO.