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. 2022 Jul 5;10:854845. doi: 10.3389/fbioe.2022.854845

TABLE 1.

Primers used for quantitative PCR.

Gene name (GeneCards®)/primer name Primer sequence 5′–3′
CCL2 Fw AGA​CTA​ACC​AGA​AAC​ATC​C (Sigma KiCqStart)
CCL2 Rev ATTGATTGCATCTGGCTG (Sigma KiCqStart)
GAPDH Fwd AGC​TCA​GGC​CTC​AAG​ACC​TT
GAPDH Rev AAG​AAG​ATG​CGG​CTG​ACT​GT
ICAM1 Fwd ACCATCTACAGCTTTCCG (Sigma KiCqStart)
ICAM1 Rev TCACACTTCACTGTCACC (Sigma KiCqStart)
SELE Fwd GAG​AAT​TCA​CCT​ACA​AGT​CC (Sigma KiCqStart)
SELE Rev AGG​CTT​GAA​CAT​TTT​ACC​AC (Sigma KiCqStart)
VCAM1 (mix Fwd/Rev) Proprietary sequence (Qiagen QuantiTect®) QT00018347

CCL2, C-C motif chemokine ligand 2. GADPH, glyceraldehyde-3-phosphate dehydrogenase. ICAM1, intercellular adhesion molecule 1. SELE, Selectin E. VCAM1, vascular cell adhesion molecule 1.