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Quantitative analysis of intracellular PIPs or Rab signals in dendrites
(A) Open an image with the Fiji-ImageJ software.
(B) Convert image format to 16-bit.
(C) Draw ROI around the dendrite outlined by adjusting the Brightness/Contrast of the fluorescent signals of mCherry the volume marker.
(D) Record ROI from Step C with the ROI Manager.
(E) Measure mean intensity of PI3P in ROI.
(F) Measure mean intensity of Rab8 in ROI.
(G) Record mean intensity of PI3P or Rab8 signals in ROI.
