| Reagent | Final concentration | Amount |
|---|---|---|
| PIPES | 40 mM | 0.6 g |
| NaCl | 274 mM | 0.8 g |
| KCl | 5.4 mM | 20.1 mg |
| ddH2O | n/a | 50 mL |
| Total | n/a | 50 mL |
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